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American Society of Microbiology ABMM Sample Questions:

1. A clinical trial is evaluating the efficacy of a new antifungal agent against invasive aspergillosis. Serum galactomannan levels are being used as a biomarker to monitor the infection. A decrease in galactomannan levels during treatment would indicate:

A) Concurrent infection with a non-Aspergillus mold.
B) Development of resistance to the antifungal agent.
C) A false-positive result in the galactomannan assay.
D) Successful clearance or reduction of the Aspergillus burden.

2. What is considered the "gold standard" for routine monitoring of the lethality of steam sterilization (autoclave) cycles in healthcare facilities?

A) Performing a Bowie-Dick test at the beginning of each day for pre-vacuum sterilizers.
B) Daily check of autoclave cycle printouts for correct time, temperature, and pressure.
C) Use of chemical indicator strips placed inside each sterilized pack.
D) Regular use of biological indicators containing spores of Geobacillus stearothermophilus.

3. A patient with a history of travel to Southeast Asia presents with fever, severe headache, and altered mental status. CSF analysis reveals lymphocytic pleocytosis and elevated protein, but routine bacterial and viral cultures are negative. Serological testing of CSF is positive for IgM antibodies against Japanese encephalitis virus. The MOST likely mode of transmission for this infection is:

A) Ingestion of contaminated food or water.
B) Direct contact with infected animals.
C) Respiratory droplets from an infected individual.
D) Bite of an infected mosquito.

4. A microbiology laboratory is investigating a possible outbreak of Salmonella infection. Serological testing of patient isolates reveals they all possess the same O antigen but different H antigens. This suggests:

A) Contamination in the laboratory affecting multiple samples.
B) A common ancestral strain that has undergone flagellar antigen variation.
C) A single point source of infection with a genetically stable strain.
D) Multiple sources of infection with unrelated Salmonella strains.

5. A clinical microbiology laboratory is investigating a potential outbreak of Legionella pneumophila associated with a hospital's water system. The MOST appropriate environmental samples to collect for culture and comparison to clinical isolates would be:

A) Water samples from showers, faucets, and cooling towers.
B) Air samples from patient rooms and common areas.
C) Sputum samples from asymptomatic healthcare workers.
D) Surface swabs from frequently touched objects.

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